Catalog No: OKEH04557
Size:96T
Price: $525.00
SKU
OKEH04557
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Egf ELISA Kit (Rat) (OKEH04557) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Egf has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Egf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Egf captured in well.
ELISA Kit Range15.6-1000pg/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Egf Microplate96 Wells (12 x 8 Well Strips)
Egf Lyophilized Standard2
Sample Diluent1 x 20 mL
Egf Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Rat Pro-epidermal growth factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolEgf
Gene Full Nameepidermal growth factor
Alias Symbolspro-epidermal growth factor.
NCBI Gene Id25313
Protein NameP07522
Description of TargetPro-epidermal growth factor
Uniprot IDhttps://www.uniprot.org/uniprot/P07522
Protein Accession #NP_036974.1
Nucleotide Accession #NM_012842.1
Protein Size (# AA)1133
Molecular Weight124 KDa
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