- NCBI Gene Id:
- Protein Name:
- Pro-epidermal growth factor
- Alias Symbols:
- URG, HOMG4, EGF
- Replacement Item:
- This antibody may replace item sc-1341 from Santa Cruz Biotechnology.
- Description of Target:
- RABBIT ANTI HUMAN EGF
- Protein Size (# AA):
- ELISA, IHC-FFPE, WB
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express EGF.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express EGF.
- The immunogen for anti-EGF antibody: recombinant human EGF
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG - lyophilised
- Reconstitution and Storage:
- Reconstitution: Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add azide.
Storage: Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-EGF antibody - OASA07717
- Polyclonal IgG
- Application Info:
- ELISA : This product may be used in an indirect ELISA system or as a capture antibody in a sandwich ELISA for human EGF in combination with This product B as a detection reagent.
Immunohistology: *This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
- Additional Information:
- Histology Positive Control Tissue: Human skin
- Preservative Stabilisers: None present.
Antiserum Preparation: Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen.
Purified IgG prepared by affinity chromatography.
- Approx Protein Conc: IgG concentration 1.0mg/ml after reconstitution
Buffer Solutions: Phosphate buffered saline pH7.4
- Target Reference:
- 1. Carpenter, G. and Cohen, S. (1990) Epidermal Growth Factor*. J. Biol. Chem. 265: 7709-7712.
2. Saito, T. et al. (2004) Differential activation of Epidermal Growth Factor (EGF) Receptor downstream signaling pathways by Betacellulin and EGF. Endocrinology. 145: 4232-4243.