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EGF Antibody (OASA07717)

0.1 mg
$609.00
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Gene Symbol:
EGF
NCBI Gene Id:
1950
Protein Name:
Pro-epidermal growth factor
Swissprot Id:
P01133
Protein Accession #:
NP_001954.2
Alias Symbols:
URG, HOMG4, EGF
Replacement Item:
This antibody may replace item sc-1341 from Santa Cruz Biotechnology.
Description of Target:
RABBIT ANTI HUMAN EGF
Protein Size (# AA):
1207
Host:
Rabbit
Clonality:
Polyclonal
Application:
ELISA, IHC-FFPE, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express EGF.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express EGF.
Immunogen:
The immunogen for anti-EGF antibody: recombinant human EGF
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - lyophilised
Reconstitution and Storage:
Reconstitution: Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add azide.
Storage: Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-EGF antibody - OASA07717
Specificity:
EGF
Isotype:
Polyclonal IgG
Application Info:
ELISA : This product may be used in an indirect ELISA system or as a capture antibody in a sandwich ELISA for human EGF in combination with This product B as a detection reagent.
Immunohistology: *This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Additional Information:
Histology Positive Control Tissue: Human skin
:::
Preservative Stabilisers: None present.
Antiserum Preparation: Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen.
Purified IgG prepared by affinity chromatography.
:::
Approx Protein Conc: IgG concentration 1.0mg/ml after reconstitution
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Carpenter, G. and Cohen, S. (1990) Epidermal Growth Factor*. J. Biol. Chem. 265: 7709-7712.
2. Saito, T. et al. (2004) Differential activation of Epidermal Growth Factor (EGF) Receptor downstream signaling pathways by Betacellulin and EGF. Endocrinology. 145: 4232-4243.

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