EFNB2 Antibody (OAAF00632)

Catalog No: OAAF00632 (Formerly GWB-ASC042)

Size:100 ug

Western blot analysis of extracts from 293 cells, treated with EGF 200ng/ml 5', using EFNB1/2 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using EFNB1/2 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human EFNB1/2.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: EFNB1/2 Antibody detects endogenous levels of total EFNB1/2 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:40000
• Reference: Agnieszka Stokowski Stem, Cells, Jan 2007; 25: 156 - 164.
  Hans-Christian Aasheim, Blood, Apr 2005; 105: 2869 - 2876.
  Zhengding Su Eur, J. Biochem., May 2004; 271: 1725 - 1736.
  Zhen Xu, J. Biol. Chem., Jun 2003; 278: 24767 - 24775.

Target Info

• Gene Symbol: EFNB1|EFNB2
• Gene Full Name: ephrin B1|ephrin B2
• Alias Symbols: CFND;CFNS;EFB1;EFL3;EFL-3;ELK ligand;Elk-L;eph-related receptor tyrosine kinase ligand 2;eph-related receptor tyrosine kinase ligand 5;ephrin-B1;ephrin-B2;EPLG2;EPLG5;HTK ligand;Htk-L;HTKL;LERK2;LERK5;ligand of eph-related kinase 5.
• NCBI Gene Id: 1947|1948
• Protein Name: Ephrin-B1|Ephrin-B2
• Description of Target: Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).|Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
• Uniprot ID: P52799|P98172
• Protein Accession #: NP_004084.1
• Molecular Weight: 38 kDa