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Catalog No: OKCD01802 (Formerly GWB-KBBYQ4)
Size:96WELLS
Price: $675.00
SKU
OKCD01802
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Datasheets/ManualsPrintable datasheet for EFNA4 ELISA Kit (Human) (OKCD01802)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-104%99-105%85-93%78-91%
EDTA Plasma (n=5)89-97%80-88%81-93%94-105%
Heparin Plasma (n=5)98-105%94-101%88-101%85-92%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin A4 (EFNA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin A4 (EFNA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin A4 (EFNA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ephrin A4 (EFNA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)91-10195
EDTA Plasma (n=5)99-105102
Heparin Plasma (n=5)78-10297
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin A4 (EFNA4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin A4 (EFNA4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EFNA4 Microplate96 Wells (12 x 8 Well Strips)
EFNA4 Lyophilized Standard2 x 20 ng
100X Biotinylated EFNA4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.129 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ephrin A4 (EFNA4). No significant cross-reactivity or interference between Ephrin A4 (EFNA4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolEFNA4
Gene Full Nameephrin A4
Alias SymbolsEFL4;eph-related receptor tyrosine kinase ligand 4;ephrin-A4;EPLG4;LERK4;LERK-4;ligand of eph-related kinase 4.
NCBI Gene Id1945
Protein NameEphrin-A4
Description of TargetCell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.
Uniprot IDP52798
Protein Accession #NP_005218.1
Nucleotide Accession #NM_005227.2
Protein Size (# AA)201
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