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Catalog No: OKEH01819
Size:96T
Price: $625.00
SKU
OKEH01819
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology EFHD2 ELISA Kit (Human) (OKEH01819) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EFHD2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EFHD2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EFHD2 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
EFHD2 Microplate96 Wells (12 x 8 Well strips)
EFHD2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated EFHD2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.2 ng/mL
SpecificityNatural and recombinant Human EF-hand domain-containing protein D2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolEFHD2
Gene Full NameEF-hand domain family member D2
Alias SymbolsEF hand domain containing 2;EF-hand domain-containing protein D2;swiprosin 1;Swiprosin-1;SWS1;testicular tissue protein Li 62.
NCBI Gene Id79180
Protein NameQ96C19
Description of TargetEF-hand domain-containing protein D2
Uniprot IDhttps://www.uniprot.org/uniprot/Q96C19
Protein Accession #NP_077305.2
Nucleotide Accession #NM_024329.5
Protein Size (# AA)240
Molecular Weight26 kDa
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