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Catalog No: OKBB01113
Size:96 wells
Price: $629.00
SKU
OKBB01113
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's mouse EFEMP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EFEMP1 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: Q18-F493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EFEMP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse EFEMP1 amount of sample captured in plate.
ELISA Kit Range312 pg/ml - 20,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)30871494546910311115004713
Standard deviation145.09821.982767.69199.1100.5353.48
CV(%)4.75.55.96.46.77.5
ELISA Kit Component
ComponentAmount
96-Well Plate Pre-coated with Anti-Mouse Efemp1 Antibody96 Wells (12 x 8 Well Strips)
Lyophilized Recombinant Mouse Efemp1 Standard2 x 10 ng
100X Biotinylated Anti-Mouse Efemp1 Antibody1 x 130 uL
100X Avidin-Biotin Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Additional Information

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 130873179309235223220178.155.5%
Sample . 21494512984150771351414130901.916.3%%
Sample 346910487405057243216473592720.135.7%

*number of samples for each test n=16.

::
Standard Number87654321
Concentration (ng/ml)01.563.136.2512.52550100
O.D.0.0900.1710.2540.3830.6581.0821.5862.236
Reconstitution and StorageStore at 4C for 6 months, at -20C for 15 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse EFEMP1
ImmunogenExpression system for standard: CHO; Immunogen sequence: Q18-F493
DescriptionFor quantitative detection of mouse EFEMP1 in cell culture supernatants, serum and plasma (heparin, EDTA).
Gene SymbolEFEMP1
Gene Full Nameepidermal growth factor-containing fibulin-like extracellular matrix protein 1
Alias SymbolsEGF-containing fibulin-like extracellular matrix protein 1; Fibulin-3; FIBL-3; Efemp1; Fbln3
NCBI Gene Id216616
Protein NameEGF-containing fibulin-like extracellular matrix protein 1
Description of TargetEGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.
Uniprot IDQ8BPB5
Protein Accession #NP_666127.2
Nucleotide Accession #NM_146015.2
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