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Catalog No: OKEH07773
Size:96T
Price: $725.00
SKU
OKEH07773
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)102-112% (n = 20)92-102% (n = 20)92-101% (n = 20)107-116% (n = 20)
EDTA plasma(n=5)116-125% (n = 20)97-107% (n = 20)88-97% (n = 20)99-109% (n = 20)
heparin plasma(n=5)85-95% (n = 20)106-116% (n = 20)89-101% (n = 20)94-106% (n = 20)
ELISA Kit PrincipleAviva Systems Biology EFEMP1 ELISA Kit (Human) (OKEH07773) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EFEMP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EFEMP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EFEMP1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 82% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=4.2% (n = 20)
Mean Inter-assay CV%: <=8.0% (n = 20)
ELISA Kit Component
ComponentAmount
EFEMP1 Microplate96 Wells (12 x 8 Well strips)
EFEMP1 Lyophilized Standard2
100X Biotinylated EFEMP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
::Subcellular Location: Secreted Extracellular space Secreted Extracellular space Extracellular matrix Localizes to the lamina propria underneath the olfactory epithelium.
::Unigene: Hs.732348
SMR: Q12805
String: 9606.ENSP00000347596
::KEGG: hsa:2202
MIM: 126600
Pfam: PF12662
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.046ng/mL
SpecificityNatural and recombinant Human EGF-containing fibulin-like extracellular matrix protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolEFEMP1
Gene Full NameEGF containing fibulin extracellular matrix protein 1
Alias SymbolsDHRD;DRAD;EGF containing fibulin like extracellular matrix protein 1;EGF-containing fibulin-like extracellular matrix protein 1;extracellular protein S1-5;FBLN3;FBNL;FIBL-3;Fibrillin-like protein;fibulin-3;MLVT;MTLV;S1-5.
NCBI Gene Id2202
Protein NameQ12805
Description of TargetEGF-containing fibulin-like extracellular matrix protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q12805
Protein Accession #NP_001034437.1
Nucleotide Accession #NM_001039348.2
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