EFEMP1 Chemi-Luminescent ELISA Kit (Human) (OKCD03450)

Catalog No: OKCD03450

Size:96WELLS

Human EFEMP1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	79-95%	98-105%	90-101%	86-97%
  EDTA Plasma (n=5)	91-99%	97-105%	88-102%	86-99%
  Heparin Plasma (n=5)	80-101%	84-94%	98-105%	87-98%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Fibulin 3 (FBLN3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibulin 3 (FBLN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibulin 3 (FBLN3) level in the sample or standard.
• ELISA Kit Range: 0.14-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96-105	101
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma (n=5)	86-101	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibulin 3 (FBLN3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibulin 3 (FBLN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • EFEMP1 Microplate: 96 Wells (12 x 8 Well strips)
  • EFEMP1 Lyophilized Standard: 2
  • 100X EFEMP1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 1.563 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EFEMP1
• Gene Full Name: EGF containing fibulin extracellular matrix protein 1
• Alias Symbols: DHRD;DRAD;EGF containing fibulin like extracellular matrix protein 1;EGF-containing fibulin-like extracellular matrix protein 1;extracellular protein S1-5;FBLN3;FBNL;FIBL-3;Fibrillin-like protein;fibulin-3;MLVT;MTLV;S1-5.
• NCBI Gene Id: 2202
• Protein Name: EGF-containing fibulin-like extracellular matrix protein 1
• Description of Target: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
• Uniprot ID: Q12805
• Protein Accession #: NP_001034437
• Nucleotide Accession #: NM_001039348