EFEMP1 Antibody (OAPB00902)

Catalog No: OAPB00902

Size:100UG

Western blot analysis of Fibulin 3 in HeLa cell lysate with Fibulin 3 antibody at (A) 0.5 and (B) 1 ug/mL.

Immunocytochemistry of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. Fibulin 3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Additional Information: HeLa Cell Lysate (OCPB00181)
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: Fibulin 3 antibody can be stored at 4C, stable for 3 months. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Fibulin 3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3. The immunogen is located within amino acids 410 - 460 of Fibulin 3.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-EFEMP1 (OAPB00902) antibody is Catalog # OEPB03024
• Application Info: Fibulin 3 antibody can be used for detection of Fibulin 3 by Western blot at 0.5 - 1 ug/ml. (Optimal dilution should be determined by user)
• Other Applications Image 1 Data: Immunocytochemistry of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 ug/mL.
• Reference: Kobayashi N, Kostka G, Garbe JH, et al. A comparative analysis of the fibulin protein family. Biochemical characterization, binding interactions, and tissue localization. J. Biol. Chem.2007; 282:11805-16.;Marmorstein LY, Munier FL, Arsenijevic Y, et al. Aberrant accumulation of EFEMP1 underlies drusen formation in Malattia Leventinese and age-related macular degeneration. Proc. Natl. Acad. Sci. USA2002; 99:13067-72.;McLaughlin PJ, Bakall B, Choi J, et al. Lack of fibulin-3 causes early aging and herniation, but not macular degeneration in mice. Hum. Mol. Genet.2007; 16:3059-70.;Seeliger H, Camaj P, Ischenko I, et al. EFEMP1 expression promotes in vivo tumor growth in human pancreatic adenocarcinoma. Mol. Cancer Res.2009; 7:189-98.

Target Info

• Gene Symbol: EFEMP1
• Gene Full Name: EGF containing fibulin extracellular matrix protein 1
• Alias Symbols: DHRD;DRAD;EGF containing fibulin like extracellular matrix protein 1;EGF-containing fibulin-like extracellular matrix protein 1;extracellular protein S1-5;FBLN3;FBNL;FIBL-3;Fibrillin-like protein;fibulin-3;MLVT;MTLV;S1-5.
• NCBI Gene Id: 2202
• Protein Name: EGF-containing fibulin-like extracellular matrix protein 1|EGF-containing fibulin-like extracellular matrix protein 1 precursor [Homo sapiens]
• Description of Target: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
• Uniprot ID: Q12805
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001034437
• Nucleotide Accession #: NM_001039348.2; NM_001039349.2
• Molecular Weight: Predicted: 52 kDa Observed: 47 kDa