EEF2K ELISA Kit (Human) (OKEH07736)

Catalog No: OKEH07736

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	90-99% (n = 20)	107-117% (n = 20)	87-97% (n = 20)	107-120% (n = 20)
  EDTA plasma(n=5)	86-98% (n = 20)	110-119% (n = 20)	84-93% (n = 20)	95-105% (n = 20)
  heparin plasma(n=5)	91-101% (n = 20)	106-118% (n = 20)	105-114% (n = 20)	95-106% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology EEF2K ELISA Kit (Human) (OKEH07736) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EEF2K has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EEF2K is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EEF2K captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.7% (n = 20)
  Mean Inter-assay CV%: <=9.2% (n = 20)
• ELISA Kit Component:
  • EEF2K Microplate: 96 Wells (12 x 8 Well strips)
  • EEF2K Lyophilized Standard: 2
  • 100X Biotinylated EEF2K Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
  Unigene: Hs.498892
  SMR: O00418
  String: 9606.ENSP00000263026
  KEGG: hsa:101930123
  MIM: 606968
  Pfam: PF02816
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 39.28pg/mL
• Specificity: Natural and recombinant Human Eukaryotic elongation factor 2 kinase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EEF2K
• Gene Full Name: eukaryotic elongation factor 2 kinase
• Alias Symbols: alternative protein EEF2K;Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase;calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase;calmodulin-dependent protein kinase III;CaMKIII;eEF-2 kinase;eEF-2K;elongation factor-2 kinase;eukaroytic elongation factor 2 kinase;eukaryotic elongation factor 2 kinase;HSU93850.
• NCBI Gene Id: 29904
• Protein Name: O00418
• Description of Target: Eukaryotic elongation factor 2 kinase
• Uniprot ID: https://www.uniprot.org/uniprot/O00418
• Protein Accession #: NP_037434.1
• Nucleotide Accession #: NM_013302.3