Catalog No: OKCD00773 (Formerly GWB-KBBKV9)
Size:96 Wells
Price: $675.00
SKU
OKCD00773
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva Systems Biology EEF1G ELISA Kit (Human) (OKCD00773) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EEF1G has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EEF1G is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EEF1Gcaptured in well.
ELISA Kit Range31.25-2,000 pg/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level EEF1G were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level EEF1G were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-EEF1G Microplate96 Wells (12 x 8 Well Strips)
EEF1G Lyophilized Standard2 x 2 ng
100X Biotinylated EEF1G Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 12.4 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g). No significant cross-reactivity or interference between Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolEEF1G
Gene Full Nameeukaryotic translation elongation factor 1 gamma
Alias SymbolsEF1G, GIG35
NCBI Gene Id1937
Protein NameElongation factor 1-gamma
Description of TargetProbably plays a role in anchoring the complex to other cellular components.
Swissprot IdP26641
Protein Accession #NP_001395
Nucleotide Accession #NM_001404.4
Protein Size (# AA)437
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express EEF1G.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express EEF1G.
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