EEF1A1 ELISA Kit (Human) (OKCD08740)

Catalog No: OKCD08740

Size:96WELLS

EEF1A1 ELISA Kit (Human) (OKCD08740) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • EEF1A1 Microplate: 96 Wells (12 x 8 Well strips)
  • EEF1A1 Lyophilized Standard: 2
  • 100X EEF1A1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.055ng/mL

Target Info

• Gene Symbol: EEF1A1
• Gene Full Name: eukaryotic translation elongation factor 1 alpha 1
• Alias Symbols: CCS3;CCS-3;cervical cancer suppressor 3;CTCL tumor antigen;EE1A1;EEF-1;EEF1A;eEF1A-1;EF1A;EF1a-like protein;EF-1-alpha-1;EF-Tu;elongation factor 1 alpha subunit;elongation factor 1-alpha 1;elongation factor Tu;epididymis secretory sperm binding protein;eukaryotic elongation factor 1 A-1;eukaryotic translation elongation factor 1 alpha 1-like 14;glucocorticoid receptor AF-1 specific elongation factor;GRAF-1EF;LENG7;leukocyte receptor cluster (LRC) member 7;leukocyte receptor cluster member 7;prostate tumor-inducing protein 1;PTI1;translation elongation factor 1 alpha 1-like 14.
• NCBI Gene Id: 1915
• Protein Name: Elongation factor 1-alpha 1
• Description of Target: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
• Uniprot ID: P68104
• Protein Accession #: NP_001393.1
• Nucleotide Accession #: NM_001402.5