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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for EED Antibody (OACA06845) (OACA06845) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot |
| Reconstitution and Storage | -20°C or -80°C |
| Immunogen | Recombinant Human Polycomb protein EED protein (1-300AA) |
| Purification | Affinity Purified |
| Preservative | 0.02% sodium azide |
| Formulation | PBS with, 50% glycerol, pH7.3. |
| Reference | [1]Binding of different histone marks differentially regulates the activity and specificity of polycomb repressive complex 2 (PRC2)."Xu C., Bian C., Yang W., Galka M., Ouyang H., Chen C., Qiu W., Liu H., Jones A.E., MacKenzie F., Pan P., Li S.S., Wang H., Min J.Proc. Natl. Acad. Sci. U.S.A. 107:19266-19271(2010). [2]System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.Sci. Signal. 4:RS3-RS3(2011). [3]Corepressor protein CDYL functions as a molecular bridge between polycomb repressor complex 2 and repressive chromatin mark trimethylated histone lysine 27." Zhang Y., Yang X., Gui B., Xie G., Zhang D., Shang Y., Liang J.J. Biol. Chem. 286:42414-42425(2011). |
| Gene Symbol | EED |
|---|---|
| Gene Full Name | embryonic ectoderm development |
| Alias Symbols | COGIS;embryonic ectoderm development protein;HEED;polycomb protein EED;WAIT1;WD protein associating with integrin cytoplasmic tails 1. |
| NCBI Gene Id | 8726 |
| Protein Name | Polycomb protein EED |
| Description of Target | Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. |
| Uniprot ID | O75530 |
| Protein Accession # | NP_001294936 |
| Nucleotide Accession # | NM_001308007 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EED Antibody (OACA06845)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "EED Antibody (OACA06845)"?
This item is available "Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks".
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What buffer format is "EED Antibody (OACA06845)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EED Antibody (OACA06845)"?
This target may also be called "COGIS;embryonic ectoderm development protein;HEED;polycomb protein EED;WAIT1;WD protein associating with integrin cytoplasmic tails 1." in publications.
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What is the shipping cost for "EED Antibody (OACA06845)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EED Antibody (OACA06845)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EED Antibody (OACA06845)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EED Antibody (OACA06845)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EED"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EED"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EED"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EED"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EED"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EED"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
