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Datasheets/Manuals | Printable datasheet for anti-Eed (ARP35696_P050-HRP) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
Peptide Sequence | Synthetic peptide located within the following region: AKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKS |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-Eed (ARP35696_P050-HRP) antibody is Catalog # AAP35696 |
Gene Symbol | Eed |
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Gene Full Name | Embryonic ectoderm development |
Alias Symbols | l7, l(7), lusk, l7Rn5, l(7)5Rn |
NCBI Gene Id | 13626 |
Protein Name | Polycomb protein EED |
Description of Target | Eed is a polycomb group (PcG) protein. It is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Eed plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Eed is required for stable maintenance of XCI in both embryonic and extra-embryonic tissues. Eed may prevent transcriptional activation of facultative heterochromatin during differentiation. Eed is required for development of secondary trophoblast giant cells during placental development. Eed may regulate hippocampal synaptic plasticity in the developing brain. |
Uniprot ID | Q921E6 |
Protein Accession # | NP_068676 |
Nucleotide Accession # | NM_021876 |
Protein Size (# AA) | 441 |
Molecular Weight | 50kDa |
Protein Interactions | Ezh2; Suz12; Rab3gap1; Ddx47; Nop10; Nip7; Bola2; Tmem14c; Uqcr10; Eif3f; Cbwd1; Smc5; Etf1; Svil; Fbxo11; Srsf7; Safb; Safb2; Abcf1; Bag6; C330027C09Rik; Pak2; Pphln1; Ttll12; Sun2; Tomm22; Eif3l; E430025E21Rik; Eny2; Dcaf13; Trio; Ccar2; Fermt2; Vps41; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Eed Antibody - N-terminal region : HRP (ARP35696_P050-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish".
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How long will it take to receive "Eed Antibody - N-terminal region : HRP (ARP35696_P050-HRP)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "Eed Antibody - N-terminal region : HRP (ARP35696_P050-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Eed Antibody - N-terminal region : HRP (ARP35696_P050-HRP)"?
This target may also be called "l7, l(7), lusk, l7Rn5, l(7)5Rn" in publications.
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What is the shipping cost for "Eed Antibody - N-terminal region : HRP (ARP35696_P050-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Eed Antibody - N-terminal region : HRP (ARP35696_P050-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Eed Antibody - N-terminal region : HRP (ARP35696_P050-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "50kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Eed Antibody - N-terminal region : HRP (ARP35696_P050-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EED"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EED"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EED"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EED"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EED"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EED"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.