EDNRA ELISA Kit (Human) (OKCA01223)

Catalog No: OKCA01223

Size:96 Wells

Human EDNRA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	103	96-107
  1:2	100	91-104
  1:4	88	81-92
  1:8	94	84-98

• ELISA Kit Principle: Aviva Systems Biology EDNRA ELISA Kit (Human) (OKCA01223) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EDNRA has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EDNRA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EDNRA captured in the well.
• ELISA Kit Range: 47 - 3,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	91	84-96
  EDTA Plasma (n=4)	102	93-106

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01223 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01223 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • EDNRA Microplate: 96 Wells (12 x 8 Well strips)
  • EDNRA Lyophilized Standard: 2 x 3 ng
  • 100X Biotinylated EDNRA Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 11.75 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: EDNRA
• Gene Full Name: Endothelin-1 receptor
• Alias Symbols: eta, ETAR
• NCBI Gene Id: 443465
• Protein Name: Endothelin-1 receptor
• Description of Target: Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.
• Uniprot ID: Q95L55
• Protein Accession #: NP_001009433.1
• Nucleotide Accession #: NM_001009433.1