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EDN3 ELISA Kit (Mouse) (OKEH01505)

96 Wells
$529.00
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Application:
ELISA
Gene Symbol:
EDN3
Official Gene Full Name:
endothelin 3
NCBI Gene Id:
13616
Alias Symbols:
114CH19, 114-CH19, Endothelin-3, ET-3, ls, PPET3, Preproendothelin-3, tmgc48
Description of Target:
This gene is a member of the endothelin family whose members encode proteins that act on G protein-coupled receptors. Endothelins are produced as large prepropolypeptide precursors that undergo a first cleavage by a subtilisin serine protease to form an inactive intermediate, which in turn is cleaved again by endothelin-converting enzyme 1 (ECE-1) to yield the active 21 amino acid peptide. This gene encodes a protein which is expressed in neural crest cells (NCC), binds to endothelin receptor b (Ednrb) and plays an essential role in the development of NCC-derived cell lineages including melanocytes and enteric neurons. Mutations in this gene are associated with terminal aganglionosis and white spotted coat in mice and Hirschsprung's disease and Waardenburg syndrome in humans.
Protein Name:
Endothelin-3
Swissprot Id:
P48299
Protein Accession #:
NP_031929.1
Nucleotide Accession #:
NM_007903.5
Predicted Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
< 10 pg/mL
Kit Range:
31.2 - 2,000 pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 6.9% (n = 20)
Mean Inter-assay CV%: < 11.8% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology EDN3 ELISA Kit (Mouse) (OKEH01505) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EDN3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EDN3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EDN3 captured in well.
Kit Component:
ComponentAmount
EDN3 Microplate96 Wells (12 x 8 Well strips)
EDN3 Lyophilized Standard2
100X Biotinylated EDN3 Detector Antibody1 x 120 µL
100X Avidin-HRP Conjugate1 x 120 µL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express EDN3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express EDN3.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural mouse ET-3. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
23 kDa
Protein Size (# AA):
214

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