EDIL3 ELISA Kit (Human) (OKCD02098)

Catalog No: OKCD02098 (Formerly GWB-KBBWH1)

Size:96WELLS

Human EDIL3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-EDIL3 Microplate: 96 Wells (12 x 8 Well Strips)
  • EDIL3 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated EDIL3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.127 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3). No significant cross-reactivity or interference between EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: EDIL3
• Gene Full Name: EGF like repeats and discoidin domains 3
• Alias Symbols: DEL1;developmental endothelial locus-1;developmentally-regulated endothelial cell locus 1 protein;EGF-like repeat and discoidin I-like domain-containing protein 3;EGF-like repeats and discoidin I-like domains 3;integrin-binding protein DEL1.
• NCBI Gene Id: 10085
• Protein Name: EGF-like repeat and discoidin I-like domain-containing protein 3
• Description of Target: Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.
• Uniprot ID: O43854
• Protein Accession #: NP_001265571.1
• Nucleotide Accession #: NM_001278642.1
• Protein Size (# AA): 480