ECT2 Recombinant Protein (OPCD02843)

Catalog No: OPCD02843

ECT2 Recombinant Protein (OPCD02843) using SDS-PAGE

ECT2 Recombinant Protein (OPCD02843) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Leu166-Arg348
  Subcellular Location: Nucleus. Cytoplasm. cytoskeleton, spindle. Cleavage furrow. Midbody. Cell junction, tight junction.
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 95%
• Source: E.coli
• Tag: N-terminal His Tag

Target Info

• Gene Symbol: ECT2
• Gene Full Name: epithelial cell transforming 2
• Alias Symbols: ARHGEF31;epithelial cell-transforming sequence 2 oncogene;protein ECT2.
• NCBI Gene Id: 1894
• Protein Name: Protein ECT2
• Description of Target: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
• Uniprot ID: Q9H8V3
• Protein Accession #: NP_001245244.1
• Nucleotide Accession #: NM_001258315.1
• Protein Size (# AA): Leu166~Arg348
• Molecular Weight: 22kDa