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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for ECT2 Antibody (OACD03154) |
|---|
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
| Reconstitution and Storage | 2°C to 8°C|-20°C |
| Immunogen | Range: Glu453 - Ile647 |
| Purification | Affinity Purified; Protein A Purified |
| Concentration | 0.5 mg/ml |
| Preservative | 0.02% NaN3 |
| Specificity | The antibody is a rabbit polyclonal antibody raised against ECT2. It has been selected for its ability to recognize ECT2 in immunohistochemical staining and western blotting. |
| Formulation | PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
| Application Data | Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5,000 Optimal working dilutions must be determined by end user. |
| Gene Symbol | ECT2 |
|---|---|
| Gene Full Name | epithelial cell transforming 2 |
| Alias Symbols | ARHGEF31;epithelial cell-transforming sequence 2 oncogene;protein ECT2. |
| NCBI Gene Id | 1894 |
| Protein Name | Protein ECT2 |
| Description of Target | Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. |
| Uniprot ID | Q9H8V3 |
| Protein Accession # | NP_001245244.1 |
| Nucleotide Accession # | NM_001258315 |
| Molecular Weight | 100 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ECT2 Antibody (OACD03154)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "ECT2 Antibody (OACD03154)"?
This item is available "Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery".
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What buffer format is "ECT2 Antibody (OACD03154)" provided in?
This item is provided in "Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ECT2 Antibody (OACD03154)"?
This target may also be called "ARHGEF31;epithelial cell-transforming sequence 2 oncogene;protein ECT2." in publications.
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What is the shipping cost for "ECT2 Antibody (OACD03154)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ECT2 Antibody (OACD03154)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ECT2 Antibody (OACD03154)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "100 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ECT2 Antibody (OACD03154)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ECT2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ECT2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ECT2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ECT2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ECT2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ECT2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
