ECO ELISA Kit (General) (OKCD01104)

Catalog No: OKCD01104 (Formerly GWB-KBBWW4)

Size:96W

General eco ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: All Species|Escherichia coli
• Application: ELISA-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ecotin (ECO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ecotin (ECO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ecotin (ECO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ecotin (ECO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ecotin (ECO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ecotin (ECO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-eco Microplate: 96 Wells (12 x 8 Well Strips)
  • eco Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated eco Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: -20°C|2°C to 8°C
• Sample Type: Biological agents
• Sensitivity: < 0.125 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Escherichia coli Protein (ECP). No significant cross-reactivity or interference between Escherichia coli Protein (ECP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: eco
• Gene Full Name: serine protease inhibitor ecotin
• Alias Symbols: b2209, ECK2201, eti, serine protease inhibitor ecotin.
• NCBI Gene Id: 946700
• Protein Name: Ecotin
• Description of Target: General inhibitor of pancreatic serine proteases: inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity.
• Uniprot ID: P23827
• Protein Accession #: NP_416713
• Nucleotide Accession #: NC_000913.3
• Protein Size (# AA): 162