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Catalog No: OKEH03903
Size:96T
Price: $625.00
SKU
OKEH03903
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ECHS1 ELISA Kit (Bovine) (OKEH03903) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ECHS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ECHS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ECHS1 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Component
ComponentAmount
Anti-ECHS1 Microplate96 Wells (12 x 8 Well strips)
ECHS1 Lyophilized Standard2
100X Biotinylated ECHS1 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.33 ng/mL
SpecificityNatural and recombinant Bovine Enoyl-CoA hydratase, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolECHS1
Gene Full Nameenoyl-CoA hydratase, short chain 1
Alias Symbolsenoyl CoA hydratase, short chain, 1, mitochondrial;enoyl Coenzyme A hydratase, short chain, 1, mitochondrial;enoyl-CoA hydratase 1;enoyl-CoA hydratase, mitochondrial;SCEH;short-chain enoyl-CoA hydratase.
NCBI Gene Id281748
Protein NameQ58DM8
Description of TargetEnoyl-CoA hydratase, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/Q58DM8
Protein Accession #NP_001020377.2
Nucleotide Accession #NM_001025206.2
Protein Size (# AA)290
Molecular Weight31 kDa
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