ECH1 ELISA Kit (Human) (OKCD00401)

Catalog No: OKCD00401 (Formerly GWB-KBBCD3)

Size:96WELLS

Human ECH1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-101%	89-102%	99-105%	96-103%
  EDTA plasma(n=5)	85-96%	80-90%	98-105%	80-89%
  heparin plasma(n=5)	90-97%	80-93%	78-102%	85-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	90-104	94
  EDTA plasma(n=5)	78-103	98
  heparin plasma(n=5)	89-97	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ECH1 Microplate: 96 Wells (12 x 8 Well Strips)
  • ECH1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated ECH1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1). No significant cross-reactivity or interference between Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ECH1
• Gene Full Name: enoyl-CoA hydratase 1
• Alias Symbols: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial;delta3,5-delta2,4-dienoyl-CoA isomerase;delta3,5-delta2,4-dienoyl-coenzyme A isomerase;dienoyl-CoA isomerase;enoyl Coenzyme A hydratase 1, peroxisomal;enoyl-CoA hydratase 1, peroxisomal;epididymis secretory sperm binding protein;HPXEL;peroxisomal enoyl-CoA hydratase 1.
• NCBI Gene Id: 1891
• Protein Name: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
• Description of Target: Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
• Uniprot ID: Q13011
• Protein Accession #: NP_001389
• Nucleotide Accession #: NM_001398.2
• Protein Size (# AA): 328