ECH1 Antibody (OAGA02165)

Catalog No: OAGA02165

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
B: Hep G2
10% SDS PAGE
OAGA02165 diluted at 1:1000

ECH1 antibody [N1C3] detects ECH1 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ECH1 antibody [N1C3] (OAGA02165) diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded OV90 xenograft, using ECH1 (OAGA02165) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ECH1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: ECH1
• Gene Full Name: enoyl CoA hydratase 1, peroxisomal
• Alias Symbols: HPXEL
• NCBI Gene Id: 1891
• Description of Target: This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
• Uniprot ID: Q13011