EBF3 Antibody (OAGA05714)

Catalog No: OAGA05714

Size:100 ul

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
diluted at 1:1000

EBF3 antibody [C1C3] detects EBF3 protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EBF3 protein stained by EBF3 antibody [C1C3] diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Immunoprecipitation of EBF3 protein from U87-MG whole cell extracts using 5 ug of EBF3 antibody [C1C3] .
Western blot analysis was performed using EBF3 antibody [C1C3] .
EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human EBF3. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.34 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: EBF3
• Gene Full Name: early B cell factor 3
• Alias Symbols: COE3, OE-2, EBF-3, HADDS, O/E-2
• NCBI Gene Id: 253738
• Protein Name: transcription factor COE3
• Description of Target: This gene encodes a member of the early B-cell factor (EBF) family of DNA binding transcription factors. EBF proteins are involved in B-cell differentiation, bone development and neurogenesis, and may also function as tumor suppressors. The encoded protein inhibits cell survival through the regulation of genes involved in cell cycle arrest and apoptosis, and aberrant methylation or deletion of this gene may play a role in multiple malignancies including glioblastoma multiforme and gastric carcinoma.
• Uniprot ID: Q9H4W6
• Protein Accession #: NP_001005463.1
• Nucleotide Accession #: NM_001005463.2