Ear11 ELISA Kit (Rat) (OKEH04700)

Catalog No: OKEH04700

Size:96T

Rat Ear11 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Ear11 ELISA Kit (Rat) (OKEH04700) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ear11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ear11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ear11 captured in well.
• ELISA Kit Range: 62.5-4000pg/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Ear11 Microplate: 96 Wells (12 x 8 Well Strips)
  • Ear11 Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Ear11 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 31.28 pg/mL
• Specificity: Natural and recombinant Rat Eosinophil cationic protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LOC100361866, Rnase3
• Gene Full Name: eosinophil cationic protein-like, ribonuclease A family member 3
• Alias Symbols: EAR;Ear11;EAR-11;ECP;eosinophil cationic protein;eosinophil secondary granule ribonuclease 11;eosinophil-associated ribonuclease;eosinophil-associated, ribonuclease A family, member 11;R1;R7;ribonuclease 1;ribonuclease 3;ribonuclease 7;ribonuclease, RNase A family, 3;RNase 3.
• NCBI Gene Id: 100361866, 192264
• Protein Name: P70709
• Description of Target: Eosinophil cationic protein
• Uniprot ID: https://www.uniprot.org/uniprot/P70709
• Protein Accession #: NP_620257.1
• Nucleotide Accession #: NM_138902.1
• Protein Size (# AA): 155
• Molecular Weight: 18 kDa