EAAC1 Antibody (OASE00254)

Catalog No: OASE00254

Size:100ug

Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Clcn3 Monoclonal Antibody, Clone S258-5. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Clcn3 Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human, Rat
• Clonality: Monoclonal
• Clone: S180-41
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IP, ICC, IF
• Additional Information: Background Info: Detects ~60kDa.
  Scientific Background: SLC1A1/EAAT3/EAAC1, a member of this transporter family, is expressed throughout the body, particularly in brain, intestine, liver, heart and kidney. Functional defects of the SLC1A1 gene are found in patients of dicarboxylicamino aciduria.
  Certificate of Analysis: 5 ug was sufficient for immunoprecipitation of EAAC1 from 50ug of rat brain lysate.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 1-524 (full length) of human EAAC1
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~60kDa.
• Dilution: WB (1:1000), ICC/IF (1:100);optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Smith C. P., et al., (1994) Genomics 20:335-336. 2.     Kanai Y., and Hediger M. A. (1992) Nature 360:467-471.
• Description: SLC1A1/EAAT3/EAAC1, a member of this transporter family, is expressed throughout the body, particularly in brain, intestine, liver, heart and kidney. Functional defects of the SLC1A1 gene are found in patients of dicarboxylicamino aciduria.

Target Info

• Gene Symbol: EAAT3
• Gene Full Name: solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
• Alias Symbols: Excitatory amino-acid carrier 1, excitatory amino acid transporter 3, EAAT3, Neuronal and epithelial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 3, solute carrier family 1 member 1 and Slc1a1
• NCBI Gene Id: 6505
• Uniprot ID: P43005
• Protein Accession #: NP_004161.4