E2F6 Antibody (OAAF01781)

Catalog No: OAAF01781 (Formerly GWB-ASD056)

Size:100 ug

Western blot analysis of extracts from HepG2 cells, using E2F6 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using E2F6 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human E2F6.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: E2F6 antibody detects endogenous levels of total E2F6 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000
• Reference: Matthew J. Oberley, J. Biol. Chem., Oct 2003; 278: 42466 - 42476.
  Claire Attwooll, J. Biol. Chem., Jan 2005; 280: 1199 - 1208.
  Paloma H. Giangrande, Genes & Dev., Dec 2004; 18: 2941 - 2951.
  Jörg Storre, J. Biol. Chem., Dec 2005; 280: 41380 - 4

Target Info

• Gene Symbol: E2F6
• Gene Full Name: E2F transcription factor 6
• Alias Symbols: E2F-6;transcription factor E2F6.
• NCBI Gene Id: 1876
• Protein Name: Transcription factor E2F6
• Description of Target: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
• Uniprot ID: O75461
• Protein Accession #: NP_937987.2
• Molecular Weight: 31 kDa