E2F5 Antibody (OAAB21800)

Catalog No: OAAB21800

Size:100ul

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with E2F5 Antibody, Unconjugated (OAAB21800) at 1:200 followed by conjugation to the secondary antibody and DAB staining

RSC96 cells probed withE2F5 Antibody, Unconjugated (OAAB21800) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IF, IHC-P
• Additional Information: Function: Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.03% Proclin300 and 50% glycerol
• Formulation: 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
• Application Info: IHC-P: 1:100~1:500
  FC: 1:20~1:100

Target Info

• Gene Symbol: E2F5
• Gene Full Name: E2F transcription factor 5
• Alias Symbols: E2F-5
• NCBI Gene Id: 1875
• Description of Target: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.
• Uniprot ID: Q15329
• Protein Accession #: NP_001077057.1
• Nucleotide Accession #: NM_001083588.1
• Molecular Weight: 38 kDa