E2F5 Antibody - N-terminal region (OAAB05599)

Catalog No: OAAB05599

Size:400ul

Western blot analysis of E2F5 Antibody (N-term) in HepG2 cell lysates (35ug/lane). E2F5 (arrow) was detected using the purified Pab.

E2F5 is strongly supported by BioGPS gene expression data to be expressed in HepG2

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with E2F5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

E2F5 Antibody (N-term) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IHC
• Additional Information: Cellular Location: Nucleus.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This E2F5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the N-terminal region of human E2F5.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat, Xenopus
• Concentration: Varies by lot. See vial for concentration.
• Sample Type Confirmation:

  E2F5 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Intended Use: E2F5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:2000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: E2F5 Antibody (N-term) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: E2F5
• Alias Symbols: E2F-5
• NCBI Gene Id: 1875
• Protein Name: Transcription factor E2F5
• Description of Target: Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor- initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
• Uniprot ID: Q15329
• Protein Accession #: NP_001077057.1
• Molecular Weight: 38 kDa