E2F4 Antibody (Acetyl-Lys96) (OASG02280)

Catalog No: OASG02280

Size:100 uL

Western Blot using L-929

Western Blot using L929, Jurkat

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB
• Additional Information: Function: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
  Cellular Localization: Nucleus.
  Tissue Specificity: Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from the human E2F-4 around the acetylation site of K96.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Differentially phosphorylated in vivo.
• Specificity: Acetyl-E2F-4 (K96) Polyclonal Antibody detects endogenous levels of E2F-4 protein only when acetylated at K96.
• Application Info: WB: 1:500-1:2000
  ELISA: 1:20000

Target Info

• Gene Symbol: E2F4
• Gene Full Name: E2F transcription factor 4, p107/p130-binding
• Alias Symbols: E2F-4
• NCBI Gene Id: 1874; 104394
• Description of Target: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
• Uniprot ID: Q16254, Q8R0K9
• Molecular Weight: 43 kDa