Size:96 Wells
$709.00
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SKU
OKCD09156
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E2F3 ELISA Kit (Human) (OKCD09156)

Catalog#: OKCD09156
Domestic: within 1-2 weeks delivery International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology E2F3 ELISA Kit (Human) (OKCD09156) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for E2F3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for E2F3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample E2F3 captured in the well.
Kit Range 0.156-10ng/mL
Kit Reproducibility Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level E2F3. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level E2F3 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Component
ComponentAmount
E2F3 Microplate96 Wells (12 x 8 Well strips)
E2F3 Lyophilized Standard2
100X E2F3 HRP-Detector Anitbody1 x 120 µL
100X Avidin-HRP Conjugate1 x 120 µL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Tissue homogenates and other biological fluids.
Sensitivity < 0.072ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Gene Symbol E2F3
Official Gene Full Name E2F transcription factor 3
Alias Symbols E2F-3
NCBI Gene Id 1871
Protein Name Transcription factor E2F3
Description of Target Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Swissprot Id O00716
Protein Accession # NP_001230005.1
Nucleotide Accession # NM_001243076.2
Tissue Tool Find tissues and cell lines supported by RNA-seq analysis to express E2F3.
RNA Seq Find tissues and cell lines supported by DNA array analysis to express E2F3.
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