E2F3 Antibody - C-terminal region : HRP (ARP38089_P050-HRP)

Catalog No: ARP38089_P050-HRP

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human E2F3
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: LLQQTEDQIPSNLEGPFVNLLPPLLQEDYLLSLGEEEGISDLFDAYDLEK
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-E2F3 (ARP38089_P050-HRP) antibody is Catalog # AAP38089 (Previous Catalog # AAPP20263)
• Specificity: The immunizing peptide used to raise this antibody is 100% homologous to isoform 2 (334aa, 37kDa) of human E2F3.
• Reference: Koehrer,K., et al., (2004) The German cDNA Consortium
• Publications:

  Naga Prasad, S. V et al. Unique microRNA profile in end-stage heart failure indicates alterations in specific cardiovascular signaling networks. J. Biol. Chem. 284, 27487-99 (2009). WB, IHC, Rat, Bovine, Pig, Horse, Guinea pig, Dog, Human, Mouse, Rabbit 19641226

  Melcher, R. et al. LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability. Carcinogenesis 32, 636-42 (2011). WB, IHC, Rat, Bovine, Pig, Horse, Guinea pig, Dog, Human, Mouse, Rabbit 21297112

  Giangreco, A. A. et al. Tumor suppressor microRNAs, miR-100 and -125b, are regulated by 1,25-dihydroxyvitamin D in primary prostate cells and in patient tissue. Cancer Prev. Res. (Phila). 6, 483-94 (2013). WB, IHC, Rat, Bovine, Pig, Horse, Guinea pig, Dog, Human, Mouse, Rabbit 23503652

Target Info

• Gene Symbol: E2F3
• Gene Full Name: E2F transcription factor 3
• Alias Symbols: E2F-3
• NCBI Gene Id: 1871
• Protein Name: Putative uncharacterized protein DKFZp686C18211 EMBL CAH18140.1
• Description of Target: E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
• Uniprot ID: Q68DT0
• Protein Accession #: CAH18140
• Nucleotide Accession #: NM_001243076
• Protein Size (# AA): 465
• Molecular Weight: 49kDa
• Protein Interactions: RB1; TRIM28; TFDP1; HDAC1; SRA1; RBL1; FZR1; ELAVL1; PCNA; MCM4; HELLS; CCNB1; BCL6; ATAD2; HCFC1; USF1; KMT2D; RYBP; TFDP2; YY1; TFE3; SP1; CCNA2; CDK3; RNF144A; FHL2; WNK1; MGA; TEAD3; SPIB; PKIB; MYBL2; CREBBP; EAPP; JMY; PPP1R13B; TP53BP2; TP53INP1; B