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E2F1 Antibody (OAGA00989)

100 ul
$329.00
In Stock
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Predicted Species Reactivity:
Human, Mouse
Product Format:
Liquid
Clonality:
Polyclonal
Isotype:
IgG
Host:
Rabbit
Application:
ICC, IF, WB
Additional Information:
Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq]
:::
Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3
:::
Cellular Localization: Nucleus
Reconstitution and Storage:
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Replacement Item:
This antibody may replace item sc-116659 from Santa Cruz Biotechnology.
Immunogen:
Recombinant fragment corresponding to a region within amino acids 133 and 362 of E2F1 (Uniprot ID#Q01094)
Purification:
Purified by antigen-affinity chromatography.
Concentration:
1 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-E2F1 antibody - OAGA00989
Application Info:
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Storage Buffer:
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Gene Symbol:
E2F1
Official Gene Full Name:
Transcription factor E2F1
Alias Symbols:
E2F-1 antibody, RBAP1 antibody, RBBP3 antibody, RBP3 antibody, E2F1 antibody, retinoblastoma-binding protein 3 antibody, RBAP-1 antibody, PRB-binding protein E2F-1 antibody, RBBP-3 antibody, transcription factor E2F1 antibody, PBR3 antibody, retinoblastom
NCBI Gene Id:
1869
Protein Name:
Transcription factor E2F1
Swissprot Id:
Q01094
Protein Accession #:
NP_005216.1
Molecular Weight:
47 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express E2F1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express E2F1.

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