E2F1 Antibody - N-terminal region : FITC (P100821_P050-FITC)

Catalog No: P100821_P050-FITC

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: WB
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human E2F1
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: PARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWA
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-E2F1 (P100821_P050-FITC) antibody is Catalog # AAP31170 (Previous Catalog # AAPP01913)
• Sample Type Confirmation:

  E2F1 is strongly supported by BioGPS gene expression data to be expressed in HEK293T

• Reference: Hu,Y., (2008) Biochem. Biophys. Res. Commun. 370 (3), 440-444

Target Info

• Gene Symbol: E2F1
• Gene Full Name: E2F transcription factor 1
• Alias Symbols: RBP3, E2F-1, RBAP1, RBBP3
• NCBI Gene Id: 1869
• Protein Name: Transcription factor E2F1
• Description of Target: The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: Q01094
• Protein Accession #: NP_005216
• Nucleotide Accession #: NM_005225
• Protein Size (# AA): 437
• Molecular Weight: 47kDa
• Protein Interactions: RB1; SIRT1; KDM2A; LAMTOR5; EP300; KAT2B; VHL; RARA; STAT1; NCOR2; STOML1; RBL1; MAGEA11; SKP2; UHRF2; UBC; GTF2H1; ERCC3; SPT15; TFB1; TBP; NCOA3; CEBPE; PARP1; PRMT2; RBL2; CCNE1; TFDP2; TFDP1; CDKN2A; CEBPD; CDK2; CDK1; NEDD8; MCPH1; DDB2; DDB1; XIAP;