DYSF ELISA Kit (Human) (OKCD00759)

Catalog No: OKCD00759 (Formerly GWB-KBBFJ2)

Size:96WELLS

Human DYSF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dysferlin (DYSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dysferlin (DYSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dysferlin (DYSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dysferlin (DYSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dysferlin (DYSF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dysferlin (DYSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DYSF Microplate: 96 Wells (12 x 8 Well Strips)
  • DYSF Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated DYSF Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 12.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dysferlin (DYSF). No significant cross-reactivity or interference between Dysferlin (DYSF) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DYSF
• Gene Full Name: dysferlin
• Alias Symbols: dysferlin;dystrophy-associated fer-1-like 1;Dystrophy-associated fer-1-like protein;FER1L1;fer-1-like family member 1;fer-1-like protein 1;LGMD2B;LGMDR2;limb girdle muscular dystrophy 2B (autosomal recessive);MMD1.
• NCBI Gene Id: 8291
• Protein Name: Dysferlin
• Description of Target: Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity).
• Uniprot ID: O75923
• Protein Accession #: NP_001123927
• Nucleotide Accession #: NM_001130455.1
• Protein Size (# AA): 2080