DYNLL1 Recombinant Antibody [20A1] (OACA12292)

Catalog No: OACA12292

Size:100UL

Overlay Peak curve showing MCF-7 cells stained with OACA12292 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of OACA12292 diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.58% DAB.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Recombinant Monoclonal
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay,
  Flow cytometry,
  Immunohistochemistry
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: A synthesized peptide derived from Human DYNLL1
• Purification: Affinity Purified

Target Info

• Gene Symbol: DYNLL1
• Gene Full Name: dynein light chain LC8-type 1
• Alias Symbols: 8 kDa dynein light chain;cytoplasmic dynein light polypeptide;DLC1;DLC8;DNCL1;DNCLC1;dynein light chain 1, cytoplasmic;Dynein light chain LC8-type 1;dynein, cytoplasmic, light polypeptide 1;hdlc1;LC8;LC8a;PIN;protein inhibitor of neuronal nitric oxide synthase.
• NCBI Gene Id: 8655
• Protein Name: Dynein light chain 1, cytoplasmic
• Description of Target: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.
• Uniprot ID: P63167