DYNC2H1 ELISA Kit (Human) (OKCD01986)

Catalog No: OKCD01986 (Formerly GWB-KBBBP8)

Size:96WELLS

Human DYNC2H1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DYNC2H1 Microplate: 96 Wells (12 x 8 Well Strips)
  • DYNC2H1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated DYNC2H1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1). No significant cross-reactivity or interference between Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DYNC2H1
• Gene Full Name: Dynein cytoplasmic 2 heavy chain 1
• Alias Symbols: ATD3, DHC2, DHC1b, DNCH2, DYH1B, SRTD3, SRPS2B, hdhc11
• NCBI Gene Id: 79659
• Protein Name: Cytoplasmic dynein 2 heavy chain 1
• Description of Target: May function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis. May play a role in transport between endoplasmic reticulum and Golgi or organization of the Golgi in cells.
• Uniprot ID: Q8NCM8
• Protein Accession #: NP_001073932.1
• Nucleotide Accession #: NM_001080463.1
• Protein Size (# AA): 4307