DYNC1I2 Antibody (OAGA00967)

Catalog No: OAGA00967

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
OAGA00967 diluted at 1:1000

DYNC1I2 antibody detects DYNC1I2 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DYNC1I2 antibody (OAGA00967) dilution: 1:500.

DYNC1I2 antibody detects DYNC1I2 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DYNC1I2 antibody (OAGA00967) dilution: 1:500.

Immunofluorescence analysis of methanol-fixed HeLa, using DYNC1I2(OAGA00967) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC, WB
• Additional Information: Background: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.
  Positive Controls: HeLa
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 129 and 366 (Q13409) of DYNC1I2
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.3 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: DYNC1I2
• Gene Full Name: Cytoplasmic dynein 1 intermediate chain 2
• Alias Symbols: IC2, DIC74, DNCI2, NEDMIBA
• NCBI Gene Id: 1781
• Protein Name: Cytoplasmic dynein 1 intermediate chain 2
• Uniprot ID: Q13409
• Protein Accession #: NP_001369.1
• Molecular Weight: 71 kDa