Catalog No: OAPC00154 (Formerly GWB-A0A333)
Size:100 ul
Price: $390.00
SKU
OAPC00154
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for OAPC00154
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Cow, Dog
Product FormatLiquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
ClonalityPolyclonal
HostSheep
ApplicationWB
Additional InformationPackaging :
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution and StorageFor long term storage -20C is recommended. Stable at -20C for at least 1 year.
ImmunogenPhosphopeptide corresponding to amino acid residues surrounding the phospho-Ser778 of rat dynamin.
PurificationPrepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Predicted Homology Based on Immunogen SequenceCow; Dog; Human; Mouse; Rat
SpecificitySpecific for the ~95k dynamin protein phosphorylated at Ser778. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by lambda -phosphatase treatment.
Application InfoThe antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda -Ptase). The blot is identical to the control except that it was incubated in lambda -Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment with lambda -Ptase.
ReferenceCousin MA, Tan TC, Robinson PJ (2001) Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. J Neurochem 76:105-116.
Graham ME, O'Callaghan DW, McMahon HT, Burgoyne RD (2002) Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size. Proc Natl Acad Sci USA 99:7124-7129.
Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ (2003) cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol 5:701-710.
Tsuboi T, Terakawa S, Scalettar BA, Fantus C, Roder J, Jeromin A (2002) Sweeping model of dynamin activity - Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins. J Biol Chem 277:15957-15961.
Gene SymbolDNM1
Alias SymbolsDnm
NCBI Gene Id140694
Protein NameDynamin-1
Description of TargetDynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
Uniprot IDP21575
Protein Accession #NP_542420.1
Molecular Weight~95kDa
  1. What is the species homology for "Dynamin Antibody (Phospho-Ser778) (OAPC00154)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog".

  2. How long will it take to receive "Dynamin Antibody (Phospho-Ser778) (OAPC00154)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Dynamin Antibody (Phospho-Ser778) (OAPC00154)" provided in?

    This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Dynamin Antibody (Phospho-Ser778) (OAPC00154)"?

    This target may also be called "Dnm" in publications.

  5. What is the shipping cost for "Dynamin Antibody (Phospho-Ser778) (OAPC00154)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Dynamin Antibody (Phospho-Ser778) (OAPC00154)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Dynamin Antibody (Phospho-Ser778) (OAPC00154)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "~95kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Dynamin Antibody (Phospho-Ser778) (OAPC00154)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "DNM1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "DNM1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "DNM1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "DNM1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "DNM1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "DNM1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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