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Catalog No: OCOA01280
Size:20UG
Price: $179.00
SKU
OCOA01280
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DVL2 Protein Lysate (OCOA01280)

Datasheets/ManualsPrintable datasheet for DVL2 Protein Lysate (OCOA01280)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionDVL2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolDVL2
Gene Full Namedishevelled segment polarity protein 2
Alias Symbolsdishevelled 2 (homologous to Drosophila dsh);dishevelled, dsh homolog 2;DSH homolog 2;segment polarity protein dishevelled homolog DVL-2.
NCBI Gene Id1856
Protein NameNM_004422, NP_004413, O14641
Description of TargetHomo sapiens dishevelled segment polarity protein 2 (DVL2), mRNA, Segment polarity protein dishevelled homolog DVL-2, segment polarity protein dishevelled homolog DVL-2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/O14641
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_004413
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_004422
Molecular Weight78.9 kDa
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