DVL2 Antibody (OAGA03728)

Catalog No: OAGA03728

Size:100 ul

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3] diluted at 1:5000 and competitor's antibody (SC-13974) diluted by 1:200.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dishevelled 2 antibody [N1N3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dishevelled 2 antibody [N1N3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3] diluted at 1:10000 and competitor's antibody (sc-13974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Dvl-2 antibody [N1N3] detects Dvl-2 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dvl-2 protein stained by Dvl-2 antibody [N1N3] diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Dvl-2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:1000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: DVL2
• Gene Full Name: dishevelled segment polarity protein 2
• NCBI Gene Id: 1856
• Protein Name: segment polarity protein dishevelled homolog DVL-2
• Description of Target: This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans.
• Uniprot ID: O14641
• Protein Accession #: NP_004413.1
• Nucleotide Accession #: NM_004422.2