DUX4 Antibody (OASE00138)

Catalog No: OASE00138 (Formerly GWB-WO9BB2)

Size:100ug

Western Blot analysis of Human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Tsp23 protein using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A. Tissue: Heart Skeletal Muscle. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone: P2B1
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF
• Additional Information: Background Info: This antibody recognizes human DUX4. Iut does not cross react with DUX4c.
  Scientific Background: DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain
  transcription factor 1 (1). Clinically it is a
  facioscapulohumeral muscular dystrophy candidate gene
  that appears to have a toxic gain of function (2-4). In
  FSHD individuals, the expression of the full-length DUX4
  transcript is not completely suppressed in skeletal muscle
  and possibly other differentiated tissues (5).
  Certificate of Analysis: 1ug/mL was sufficient for detection of DUX4 in 20ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~45kDa. No cross-reactivity with DUX4c.
• Dilution: WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Entrez Gene: “Dux4 Double Homeobox, 4” 2. Dixit M., et al., (2007) Proc. Natl Acad Sci. USA.
  104(46): 18157-18162.
  3. Kowaljow V., et al. (2007) Neuromuscl Disord. 17(8):
  611-623.
  4. Lemmers R., et al. (2010) Science Express. 329(5999):
  1650-1653.
  5. Snider L., et al. (2010) PLoS Genetics. 6:1-14.
• Description: DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain transcription factor 1 (1). Clinically it is a facioscapulohumeral muscular dystrophy candidate gene that appears to have a toxic gain of function (2-4). In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle and possibly other differentiated tissues (5).

Target Info

• Gene Symbol: DUX4
• Gene Full Name: double homeobox 4
• Alias Symbols: Double homeobox 4, Double homeobox 10, DUX10, Double homeobox protein 4/10, DUX4L, DUX4L1
• NCBI Gene Id: 22947
• Uniprot ID: F5GZ66
• Protein Accession #: NP_149418.3