DUX4 Antibody : FITC (OASE00138-FITC)

Catalog No: OASE00138-FITC

Size:100ug

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: P2B1
• Isotype: IgG1
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: WB, IF, IHC
• Additional Information: Background Info: This antibody recognizes human DUX4. Iut does not cross react with DUX4c.
  Scientific Background: DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain
  transcription factor 1 (1). Clinically it is a
  facioscapulohumeral muscular dystrophy candidate gene
  that appears to have a toxic gain of function (2-4). In
  FSHD individuals, the expression of the full-length DUX4
  transcript is not completely suppressed in skeletal muscle
  and possibly other differentiated tissues (5).
  Certificate of Analysis: 1ug/mL was sufficient for detection of DUX4 in 20ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Entrez Gene: “Dux4 Double Homeobox, 4” 2. Dixit M., et al., (2007) Proc. Natl Acad Sci. USA.
  104(46): 18157-18162.
  3. Kowaljow V., et al. (2007) Neuromuscl Disord. 17(8):
  611-623.
  4. Lemmers R., et al. (2010) Science Express. 329(5999):
  1650-1653.
  5. Snider L., et al. (2010) PLoS Genetics. 6:1-14.

Target Info

• Gene Symbol: DUX4
• Gene Full Name: double homeobox 4
• Alias Symbols: DUX4, DUX10
• NCBI Gene Id: 22947
• Uniprot ID: F5GZ66
• Protein Accession #: NP_149418.3