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Catalog No: OKCD08868
Size:96WELLS
Price: $800.00
SKU
OKCD08868
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Datasheets/ManualsPrintable datasheet for DUSP5 ELISA Kit (Human) (OKCD08868)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Phosphatase 5 (DUSP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Phosphatase 5 (DUSP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Phosphatase 5 (DUSP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dual Specificity Phosphatase 5 (DUSP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Specificity Phosphatase 5 (DUSP5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Specificity Phosphatase 5 (DUSP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
DUSP5 Microplate96 Wells (12 x 8 Well strips)
DUSP5 Lyophilized Standard2
100X DUSP5 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.053ng/mL
Gene SymbolDUSP5
Gene Full Namedual specificity phosphatase 5
Alias Symbolsdual specificity protein phosphatase 5;dual specificity protein phosphatase hVH3;DUSP;HVH3;serine/threonine specific protein phosphatase;VH1-like phosphatase 3.
NCBI Gene Id1847
Protein NameDual specificity protein phosphatase 5
Description of TargetDual specificity protein phosphatase; active with phosphotyrosine, phosphoserine and phosphothreonine residues. The highest relative activity is toward ERK1.
Uniprot IDQ16690
Protein Accession #NP_004410.3
Nucleotide Accession #NM_004419.3
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