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Catalog No: OKEH02215
Size:96T
Price: $525.00
SKU
OKEH02215
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DUSP3 ELISA Kit (Human) (OKEH02215) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DUSP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DUSP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DUSP3 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
DUSP3 Microplate96 Wells (12 x 8 Well strips)
DUSP3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated DUSP3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.058 ng/mL
SpecificityNatural and recombinant Human Dual specificity protein phosphatase 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDUSP3
Gene Full Namedual specificity phosphatase 3
Alias Symbolsdual specificity protein phosphatase 3;dual specificity protein phosphatase VHR;serine/threonine specific protein phosphatase;vaccinia H1-related phosphatase;vaccinia virus phosphatase VH1-related;VHR.
NCBI Gene Id1845
Protein NameP51452
Description of TargetDual specificity protein phosphatase 3
Uniprot IDhttps://www.uniprot.org/uniprot/P51452
Protein Accession #NP_004081.1
Nucleotide Accession #NM_004090.3
Protein Size (# AA)185
Molecular Weight20 kDa
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