DUSP3 Antibody (OAGA00742)

Catalog No: OAGA00742

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
OAGA00742 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse stomach
12% SDS PAGE
OAGA00742 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using DUSP3(OAGA00742) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq]
  Positive Controls: 293T, HeLaS3, HepG2, mouse stomach
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 185 of DUSP3 (Uniprot ID#P51452)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.58 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: DUSP3
• Gene Full Name: Dual specificity protein phosphatase 3
• Alias Symbols: VHR
• NCBI Gene Id: 1845
• Protein Name: Dual specificity protein phosphatase 3
• Uniprot ID: P51452
• Protein Accession #: NP_004081.1
• Molecular Weight: 20 kDa