Catalog No: OKCD02508
Size:96WELLS
Price: $700.00
SKU
OKCD02508
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Datasheets/ManualsPrintable datasheet for DUSP1 ELISA Kit (Mouse) (OKCD02508)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)98-105%80-97%91-99%98-105%
EDTA Plasma (n=5)81-99%78-94%80-91%78-93%
Heparin Plasma (n=5)80-103%91-102%91-99%95-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Phosphatase 1 (DUSP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Phosphatase 1 (DUSP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Phosphatase 1 (DUSP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dual Specificity Phosphatase 1 (DUSP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)87-10196
EDTA Plasma (n=5)88-9592
Heparin Plasma(n=5)92-10295
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Specificity Phosphatase 1 (DUSP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Specificity Phosphatase 1 (DUSP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DUSP1 Microplate96 Wells (12 x 8 Well strips)
DUSP1 Lyophilized Standard2
100X Biotinylated DUSP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity13.2 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dual Specificity Phosphatase 1 (DUSP1). No significant cross-reactivity or interference between Dual Specificity Phosphatase 1 (DUSP1) and analogues was observed
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolDusp1
Gene Full Namedual specificity phosphatase 1
Alias Symbols3CH134;dual specificity protein phosphatase 1;e;erp;MAP kinase phosphatase 1;Mitogen-activated protein kinase phosphatase 1;mitogen-activated protein kinase phosphatase-1;MKP;mkp-;mkp-1;MKP1;protein tyrosine phosphatase, non-receptor type 16;protein-tyrosine phosphatase 3CH134;protein-tyrosine phosphatase ERP;Ptp;Ptpn16;U19515.
NCBI Gene Id19252
Protein NameDual specificity protein phosphatase 1
Description of TargetDual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
Uniprot IDP28563
Protein Accession #NP_038670.1
Nucleotide Accession #NM_013642.3
Protein Size (# AA)367
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