DUSP1 Antibody (Phospho-Ser296/318) (OASG04554)

Catalog No: OASG04554

Size:100 uL

Western Blot using JK-1

Western Blot

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human MKP-1/2 around the phosphorylation site of S296/318. Location: 230-310aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Ser296/318
• Specificity: Phospho-MKP-1/2 (S296/318) Polyclonal Antibody detects endogenous levels of MKP-1/2 protein only when phosphorylated at S296/318.
• Application Info: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Target Info

• Gene Symbol: DUSP1, DUSP4
• Gene Full Name: dual specificity phosphatase 1
• Alias Symbols: HVH1, MKP1, CL100, MKP-1, PTPN10
• NCBI Gene Id: 1843; 1846; 19252; 319520; 114856; 60587
• Description of Target: The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
• Uniprot ID: P28562, Q13115, P28563, Q8BFV3, Q64623, Q62767
• Molecular Weight: 45 kDa