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Catalog No: OKCD02397
Size:96WELLS
Price: $725.00
SKU
OKCD02397
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Datasheets/ManualsPrintable datasheet for DUOX2 ELISA Kit (Rat) (OKCD02397)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)80-96%79-92%83-91%91-103%
EDTA Plasma (n=5)79-90%82-93%82-97%88-102%
Heparin Plasma (n=5)96-105%87-95%82-101%81-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Oxidase 2 (DUOX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Oxidase 2 (DUOX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Oxidase 2 (DUOX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dual Oxidase 2 (DUOX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-9895
EDTA Plasma (n=5)78-9788
Heparin Plasma(n=5)80-9483
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Oxidase 2 (DUOX2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Oxidase 2 (DUOX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DUOX2 Microplate96 Wells (12 x 8 Well strips)
DUOX2 Lyophilized Standard2
100X Biotinylated DUOX2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.063 ng/mL.
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dual Oxidase 2 (DUOX2). No significant cross-reactivity or interference between Dual Oxidase 2 (DUOX2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolDuox2
Gene Full Namedual oxidase 2
Alias Symbolsdual oxidase 2;large NOX 2;long NOX 2;NADH/NADPH thyroid oxidase THOX2;Thox2;thyroid oxidase 2.
NCBI Gene Id79107
Protein NameDual oxidase 2
Description of TargetGenerates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.
Uniprot IDQ9ES45
Protein Accession #NP_077055.1
Nucleotide Accession #NM_024141.1
Protein Size (# AA)1517
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