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Catalog No: OKEH08320
Size:96T
Price: $725.00
SKU
OKEH08320
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology DUOX1 ELISA Kit (Human) (OKEH08320) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DUOX1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DUOX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DUOX1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.8% (n = 20)
Mean Inter-assay CV%: <=8.4% (n = 20)
ELISA Kit Component
ComponentAmount
DUOX1 Microplate96 Wells (12 x 8 Well strips)
DUOX1 Lyophilized Standard2
100X Biotinylated DUOX1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.
::Subcellular Location: Apical cell membrane Multi-pass membrane protein Localizes to the apical membrane of epithelial cells.
::Unigene: Hs.272813
String: 9606.ENSP00000317997
::KEGG: hsa:53905
MIM: 606758
Pfam: PF03098
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.066ng/mL
SpecificityNatural and recombinant Human Dual oxidase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDUOX1
Gene Full Namedual oxidase 1
Alias Symbolsdual oxidase 1;flavoprotein NADPH oxidase;large NOX 1;LNOX1;long NOX 1;NADPH thyroid oxidase 1;nicotinamide adenine dinucleotide phosphate oxidase;NOXEF1;THOX1;thyroid oxidase 1.
NCBI Gene Id53905
Protein NameQ9NRD9
Description of TargetDual oxidase 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9NRD9
Protein Accession #NP_059130.2
Nucleotide Accession #NM_017434.4
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