Catalog No: OKEH08320
Size:96T
Price: $725.00
SKU
OKEH08320
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology DUOX1 ELISA Kit (Human) (OKEH08320) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DUOX1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DUOX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DUOX1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=7.8% (n = 20) Mean Inter-assay CV%: <=8.4% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain. | ||||||||||||||||||||||
:: | Subcellular Location: Apical cell membrane Multi-pass membrane protein Localizes to the apical membrane of epithelial cells. | ||||||||||||||||||||||
:: | Unigene: Hs.272813 String: 9606.ENSP00000317997 | ||||||||||||||||||||||
:: | KEGG: hsa:53905 MIM: 606758 Pfam: PF03098 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.066ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Dual oxidase 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | DUOX1 |
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Gene Full Name | dual oxidase 1 |
Alias Symbols | dual oxidase 1;flavoprotein NADPH oxidase;large NOX 1;LNOX1;long NOX 1;NADPH thyroid oxidase 1;nicotinamide adenine dinucleotide phosphate oxidase;NOXEF1;THOX1;thyroid oxidase 1. |
NCBI Gene Id | 53905 |
Protein Name | Q9NRD9 |
Description of Target | Dual oxidase 1 |
Uniprot ID | https://www.uniprot.org/uniprot/Q9NRD9 |
Protein Accession # | NP_059130.2 |
Nucleotide Accession # | NM_017434.4 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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